| City | Population |
| Allamakee County rural, IA | 6,213 |
| Harpers Ferry, IA | 328 |
| Lansing, IA | 999 |
| New Albin, IA | 522 |
| Waterville, IA | 144 |
| Waukon, IA | 3,897 |
| Altura, MN | 493 |
| Brownsville, MN | 466 |
| Caledonia, MN | 2,868 |
| Dakota, MN | 323 |
| Eitzen, MN | 243 |
| Elba, MN | 152 |
| Goodview, MN | 4,036 |
| Hokah, MN | 580 |
| Homer, MN | 181 |
| Houston, MN | 979 |
| Houston County rural, MN | 7,731 |
| Kellogg, MN | 456 |
| La Crescent, MN | 4,830 |
| Lewiston, MN | 1,620 |
| Mabel, MN | 780 |
| Minneiska, MN | 111 |
| Minnesota City, MN | 204 |
| Peterson, MN | 199 |
| Rollingstone, MN | 664 |
| Rushford, MN | 1,731 |
| Rushford Village, MN | 807 |
| Spring Grove, MN | 1,330 |
| Stockton, MN | 697 |
| Utica, MN | 291 |
| Wabasha, MN | 2,521 |
| Whalan, MN | 63 |
| Winona, MN | 27,592 |
| Winona County rural, MN | 11,473 |
| Alma, WI | 781 |
| Alma Center village, WI | 503 |
| Altoona, WI | 6,706 |
| Arcadia, WI | 2,925 |
| Arkansaw, WI | 177 |
| Arkdale, WI | 158 |
| Augusta, WI | 1,550 |
| Bangor village, WI | 1,459 |
| Barron, WI | 3,423 |
| Barron County rural, WI | 24,828 |
| Bell Center village, WI | 117 |
| Birchwood village, WI | 442 |
| Black River Falls, WI | 3,622 |
| Blair, WI | 1,366 |
| Bloomer, WI | 3,539 |
| Boscobel, WI | 3,231 |
| Boyceville village, WI | 1,086 |
| Boyd village, WI | 552 |
| Brice Prairie, WI | 1,887 |
| Bruce village, WI | 779 |
| Buffalo City, WI | 1,023 |
| Buffalo County rural, WI | 7,255 |
| Cadott village, WI | 1,437 |
| Cameron village, WI | 1,783 |
| Cashton village, WI | 1,102 |
| Cataract, WI | 186 |
| Chaseburg village, WI | 284 |
| Chetek, WI | 2,221 |
| Chippewa County rural, WI | 27,913 |
| Chippewa Falls, WI | 13,661 |
| Clark County rural, WI | 23,101 |
| Cochrane village, WI | 450 |
| Colfax village, WI | 1,158 |
| Conrath village, WI | 95 |
| Coon Valley village, WI | 765 |
| Cornell, WI | 1,467 |
| Couderay village, WI | 88 |
| Crawford County rural, WI | 7,744 |
| Cumberland, WI | 2,170 |
| Dallas village, WI | 409 |
| De Soto village, WI | 287 |
| Delwood, WI | 563 |
| Dodge, WI | 121 |
| Downing village, WI | 265 |
| Downsville, WI | 146 |
| Dunn County rural, WI | 20,734 |
| Durand, WI | 1,931 |
| Eastman village, WI | 428 |
| Eau Claire, WI | 65,883 |
| Eau Claire County rural, WI | 21,314 |
| Eleva village, WI | 670 |
| Elk Mound village, WI | 878 |
| Elmwood village, WI | 817 |
| Emerald, WI | 161 |
| Ettrick village, WI | 524 |
| Exeland village, WI | 196 |
| Fairchild village, WI | 550 |
| Fall Creek village, WI | 1,315 |
| Ferryville village, WI | 176 |
| Fountain City, WI | 859 |
| French Island, WI | 4,207 |
| Galesville, WI | 1,481 |
| Gays Mills village, WI | 491 |
| Genoa village, WI | 253 |
| Gilman village, WI | 410 |
| Glen Flora village, WI | 92 |
| Glenwood City, WI | 1,242 |
| Granton village, WI | 355 |
| Greenwood, WI | 1,026 |
| Hatfield, WI | 141 |
| Haugen village, WI | 287 |
| Hawkins village, WI | 305 |
| Hixton village, WI | 433 |
| Holcombe, WI | 267 |
| Holmen village, WI | 9,005 |
| Humbird, WI | 266 |
| Hustler village, WI | 194 |
| Independence, WI | 1,336 |
| Ingram village, WI | 78 |
| Jackson County rural, WI | 14,104 |
| Jim Falls, WI | 237 |
| Jump River, WI | 52 |
| Kennan village, WI | 135 |
| Knapp village, WI | 463 |
| La Crosse, WI | 51,320 |
| La Crosse County rural, WI | 23,128 |
| La Farge village, WI | 746 |
| Ladysmith, WI | 3,414 |
| Lake Hallie village, WI | 6,448 |
| Lake Wissota, WI | 2,738 |
| Loyal, WI | 1,261 |
| Lublin village, WI | 118 |
| Lynxville village, WI | 132 |
| Melrose village, WI | 503 |
| Melvina village, WI | 104 |
| Menomonie, WI | 16,264 |
| Merrillan village, WI | 542 |
| Millston, WI | 125 |
| Mondovi, WI | 2,777 |
| Monroe County rural, WI | 22,139 |
| Mount Sterling village, WI | 211 |
| Necedah village, WI | 916 |
| Neillsville, WI | 2,463 |
| Nelson village, WI | 374 |
| New Auburn village, WI | 548 |
| Norwalk village, WI | 638 |
| Oakdale village, WI | 297 |
| Onalaska, WI | 17,736 |
| Ontario village, WI | 554 |
| Osseo, WI | 1,701 |
| Owen, WI | 940 |
| Pepin County rural, WI | 4,458 |
| Pepin village, WI | 837 |
| Pigeon Falls village, WI | 411 |
| Plum City village, WI | 599 |
| Prairie Farm village, WI | 473 |
| Radisson village, WI | 241 |
| Readstown village, WI | 415 |
| Reserve, WI | 429 |
| Rice Lake, WI | 8,438 |
| Ridgeland village, WI | 273 |
| Rockland village, WI | 594 |
| Rusk County rural, WI | 9,404 |
| Sawyer County rural, WI | 11,003 |
| Seymour, WI | 1,418 |
| Sheldon village, WI | 237 |
| Soldiers Grove village, WI | 592 |
| Sparta, WI | 9,522 |
| St. Joseph, WI | 503 |
| Stanley, WI | 3,608 |
| Steuben village, WI | 131 |
| Stoddard village, WI | 774 |
| Strum village, WI | 1,114 |
| Tainter Lake, WI | 2,242 |
| Taylor village, WI | 476 |
| Thorp, WI | 1,621 |
| Tomah, WI | 9,093 |
| Tony village, WI | 113 |
| Trempealeau County rural, WI | 14,080 |
| Trempealeau village, WI | 1,529 |
| Tunnel City, WI | 106 |
| Vernon County rural, WI | 17,017 |
| Viola village, WI | 699 |
| Viroqua, WI | 4,362 |
| Warrens village, WI | 363 |
| Waumandee, WI | 68 |
| Wauzeka village, WI | 711 |
| West Salem village, WI | 4,799 |
| Westby, WI | 2,200 |
| Weyerhaeuser village, WI | 238 |
| Wheeler village, WI | 348 |
| Whitehall, WI | 1,558 |
| Wilson village, WI | 184 |
| Wilton village, WI | 504 |
| Winter village, WI | 313 |
| Withee village, WI | 487 |
| Woodman village, WI | 132 |
| Wyeville village, WI | 147 |
| | |
| Total | 670,646 |
