| City | Population |
| Argyle town, MO | 162 |
| Armstrong, MO | 284 |
| Arrow Rock town, MO | 56 |
| Ashland, MO | 3,707 |
| Audrain County rural, MO | 8,622 |
| Auxvasse, MO | 983 |
| Bagnell town, MO | 93 |
| Barnett, MO | 203 |
| Belle, MO | 1,545 |
| Benton City village, MO | 104 |
| Bevier, MO | 718 |
| Big Spring, MO | 167 |
| Blackwater, MO | 162 |
| Bland, MO | 539 |
| Boone County rural, MO | 43,320 |
| Boonville, MO | 8,319 |
| Bucklin, MO | 467 |
| Bunceton, MO | 354 |
| Cairo village, MO | 292 |
| California, MO | 4,278 |
| Callao, MO | 292 |
| Callaway County rural, MO | 25,980 |
| Centertown town, MO | 278 |
| Centralia, MO | 4,027 |
| Chamois, MO | 396 |
| Chariton County rural, MO | 4,454 |
| Clark, MO | 298 |
| Clarksburg, MO | 334 |
| Clifton Hill, MO | 114 |
| Cole County rural, MO | 27,876 |
| Columbia, MO | 108,500 |
| Cooper County rural, MO | 6,894 |
| Dalton town, MO | 17 |
| Danville, MO | 34 |
| Eldon, MO | 4,567 |
| Excello, MO | 49 |
| Farber, MO | 322 |
| Fayette, MO | 2,688 |
| Franklin, MO | 95 |
| Freeburg village, MO | 437 |
| Fulton, MO | 12,790 |
| Gasconade, MO | 223 |
| Gasconade County rural, MO | 8,805 |
| Gilliam, MO | 197 |
| Glasgow, MO | 1,103 |
| Gravois Mills town, MO | 144 |
| Hallsville, MO | 1,491 |
| Harrisburg town, MO | 266 |
| Hartsburg town, MO | 103 |
| Hermann, MO | 2,431 |
| Higbee, MO | 568 |
| Holliday village, MO | 137 |
| Holts Summit, MO | 3,247 |
| Howard County rural, MO | 4,885 |
| Hughesville village, MO | 183 |
| Huntsdale town, MO | 31 |
| Huntsville, MO | 1,564 |
| Iberia, MO | 736 |
| Jacksonville village, MO | 151 |
| Jamestown town, MO | 386 |
| Jefferson City, MO | 43,079 |
| Keytesville, MO | 471 |
| Kingdom City village, MO | 128 |
| Laddonia, MO | 513 |
| Lake Mykee Town village, MO | 350 |
| Lake Ozark, MO | 1,586 |
| Laurie, MO | 945 |
| Linn, MO | 1,459 |
| Lohman, MO | 163 |
| Lupus town, MO | 33 |
| Macon, MO | 5,471 |
| Madison, MO | 554 |
| Marceline, MO | 2,233 |
| Maries County rural, MO | 7,021 |
| Marshall, MO | 13,065 |
| Martinsburg town, MO | 304 |
| McBaine town, MO | 10 |
| McKittrick town, MO | 61 |
| Meta, MO | 229 |
| Mexico, MO | 11,543 |
| Miller County rural, MO | 17,008 |
| Moberly, MO | 13,974 |
| Mokane, MO | 185 |
| Moniteau County rural, MO | 7,314 |
| Monroe County rural, MO | 4,362 |
| Montgomery City, MO | 2,834 |
| Montgomery County rural, MO | 5,489 |
| Morgan County rural, MO | 15,525 |
| Morrison, MO | 139 |
| Nelson, MO | 192 |
| New Bloomfield, MO | 669 |
| New Cambria, MO | 195 |
| New Florence, MO | 769 |
| New Franklin, MO | 1,089 |
| Olean town, MO | 128 |
| Osage Beach, MO | 4,351 |
| Osage County rural, MO | 10,806 |
| Otterville, MO | 454 |
| Paris, MO | 1,220 |
| Pettis County rural, MO | 18,225 |
| Pierpont village, MO | 76 |
| Pilot Grove, MO | 768 |
| Prairie Home, MO | 280 |
| Randolph County rural, MO | 8,281 |
| Renick village, MO | 172 |
| Rhineland town, MO | 142 |
| Rocheport, MO | 239 |
| Rush Hill village, MO | 151 |
| Russellville, MO | 807 |
| Saline County rural, MO | 5,693 |
| Salisbury, MO | 1,618 |
| Sedalia, MO | 21,387 |
| Slater, MO | 1,856 |
| Smithton, MO | 570 |
| St. Elizabeth village, MO | 336 |
| St. James, MO | 4,216 |
| St. Martins, MO | 1,140 |
| St. Thomas town, MO | 263 |
| Stover, MO | 1,094 |
| Sturgeon, MO | 872 |
| Sunrise Beach village, MO | 431 |
| Syracuse, MO | 172 |
| Taos, MO | 878 |
| Tipton, MO | 3,262 |
| Tuscumbia town, MO | 203 |
| Vandalia, MO | 3,899 |
| Vandiver village, MO | 71 |
| Versailles, MO | 2,482 |
| Vienna, MO | 610 |
| Village of Four Seasons village, MO | 2,217 |
| Wardsville village, MO | 1,506 |
| Wellsville, MO | 1,217 |
| Westphalia, MO | 389 |
| Windsor Place village, MO | 309 |
| Wooldridge village, MO | 61 |
| | |
| Total | 563,282 |
