| City | Population |
| Adena village, OH | 759 |
| Amsterdam village, OH | 511 |
| Antioch village, OH | 86 |
| Baltic village, OH | 795 |
| Bannock, OH | 211 |
| Barnesville village, OH | 4,193 |
| Barnhill village, OH | 396 |
| Batesville village, OH | 71 |
| Beallsville village, OH | 409 |
| Bellaire village, OH | 4,278 |
| Belmont County rural, OH | 33,991 |
| Belmont village, OH | 453 |
| Bergholz village, OH | 664 |
| Bethesda village, OH | 1,256 |
| Bloomingdale village, OH | 202 |
| Bowerston village, OH | 398 |
| Bridgeport village, OH | 1,831 |
| Brilliant, OH | 1,482 |
| Brookside village, OH | 632 |
| Buffalo, OH | 401 |
| Byesville village, OH | 2,438 |
| Cadiz village, OH | 3,353 |
| Calcutta, OH | 3,742 |
| Cambridge, OH | 10,635 |
| Carroll County rural, OH | 21,936 |
| Carrollton village, OH | 3,241 |
| Clarington village, OH | 384 |
| Deersville village, OH | 79 |
| Dellroy village, OH | 356 |
| Dennison village, OH | 2,655 |
| Dillonvale village, OH | 665 |
| Dover, OH | 12,826 |
| East Liverpool, OH | 11,195 |
| Empire village, OH | 299 |
| Fairview village, OH | 83 |
| Flushing village, OH | 879 |
| Freeport village, OH | 369 |
| Fresno, OH | 140 |
| Glencoe, OH | 310 |
| Glenmoor, OH | 1,987 |
| Gnadenhutten village, OH | 1,288 |
| Guernsey County rural, OH | 23,880 |
| Hannibal, OH | 411 |
| Harrison County rural, OH | 8,584 |
| Harrisville village, OH | 235 |
| Holloway village, OH | 338 |
| Hopedale village, OH | 950 |
| Irondale village, OH | 387 |
| Jefferson County rural, OH | 29,136 |
| Jerusalem village, OH | 161 |
| Jewett village, OH | 692 |
| Kimbolton, OH | 144 |
| La Croft, OH | 1,144 |
| Lafferty, OH | 304 |
| Lansing, OH | 634 |
| Leesville village, OH | 158 |
| Lewisville village, OH | 176 |
| Lore City village, OH | 325 |
| Martins Ferry, OH | 6,915 |
| Midvale village, OH | 754 |
| Miltonsburg village, OH | 43 |
| Mingo Junction village, OH | 3,454 |
| Monroe County rural, OH | 9,747 |
| Morristown village, OH | 303 |
| Mount Pleasant village, OH | 478 |
| Neffs, OH | 993 |
| New Alexandria village, OH | 272 |
| New Athens village, OH | 320 |
| New Philadelphia, OH | 17,288 |
| Newcomerstown village, OH | 3,822 |
| Noble County rural, OH | 12,054 |
| Old Washington village, OH | 279 |
| Parral village, OH | 218 |
| Pleasant City village, OH | 447 |
| Port Washington village, OH | 569 |
| Pottery Addition, OH | 293 |
| Powhatan Point village, OH | 1,592 |
| Quaker City village, OH | 502 |
| Rayland village, OH | 417 |
| Richmond village, OH | 481 |
| Roswell village, OH | 219 |
| Salesville village, OH | 129 |
| Salineville village, OH | 1,311 |
| Sarahsville village, OH | 166 |
| Sardis, OH | 559 |
| Scio village, OH | 763 |
| Senecaville village, OH | 457 |
| Shadyside village, OH | 3,785 |
| Sherrodsville village, OH | 304 |
| Smithfield village, OH | 869 |
| St. Clairsville, OH | 5,184 |
| Stafford village, OH | 81 |
| Steubenville, OH | 18,659 |
| Stone Creek village, OH | 177 |
| Stratton village, OH | 294 |
| Sugarcreek village, OH | 2,220 |
| Summerfield village, OH | 254 |
| Tiltonsville village, OH | 1,372 |
| Tippecanoe, OH | 121 |
| Toronto, OH | 5,091 |
| Tuscarawas County rural, OH | 37,723 |
| Tuscarawas village, OH | 1,056 |
| Uhrichsville, OH | 5,413 |
| Wellsville village, OH | 3,541 |
| Wilson village, OH | 125 |
| Wintersville village, OH | 3,924 |
| Wolfhurst, OH | 1,239 |
| Woodsfield village, OH | 2,384 |
| Yorkville village, OH | 1,079 |
| Claysville borough, PA | 829 |
| Greene County rural, PA | 23,537 |
| New Freeport, PA | 112 |
| Rogersville, PA | 249 |
| West Alexander, PA | 604 |
| Wind Ridge, PA | 215 |
| Beech Bottom village, WV | 523 |
| Benwood, WV | 1,420 |
| Bethany town, WV | 1,036 |
| Bethlehem village, WV | 2,499 |
| Brooke County rural, WV | 13,706 |
| Cameron, WV | 946 |
| Chester, WV | 2,585 |
| Clearview village, WV | 565 |
| Follansbee, WV | 2,986 |
| Glen Dale, WV | 1,526 |
| Hancock County rural, WV | 5,866 |
| Hooverson Heights, WV | 2,590 |
| Marshall County rural, WV | 17,971 |
| McMechen, WV | 1,926 |
| Moundsville, WV | 9,318 |
| New Cumberland, WV | 1,103 |
| New Martinsville, WV | 5,366 |
| Newell, WV | 1,376 |
| Ohio County rural, WV | 10,162 |
| Paden City, WV | 2,633 |
| Sistersville, WV | 1,396 |
| Triadelphia town, WV | 811 |
| Valley Grove village, WV | 378 |
| Weirton, WV | 19,746 |
| Wellsburg, WV | 2,805 |
| West Liberty town, WV | 1,542 |
| Wheeling, WV | 28,486 |
| Windsor Heights village, WV | 423 |
| | |
| Total | 526,514 |
