| City | Population |
| Ashland, KY | 21,684 |
| Bellefonte, KY | 888 |
| Blaine, KY | 47 |
| Boyd County rural, KY | 20,400 |
| Cannonsburg, KY | 856 |
| Carter County rural, KY | 21,904 |
| Catlettsburg, KY | 1,856 |
| Elliott County rural, KY | 7,177 |
| Flatwoods, KY | 7,423 |
| Garrison, KY | 866 |
| Grayson, KY | 4,217 |
| Greenup, KY | 1,188 |
| Greenup County rural, KY | 17,881 |
| Inez, KY | 717 |
| Johnson County rural, KY | 19,897 |
| Lawrence County rural, KY | 13,346 |
| Louisa, KY | 2,467 |
| Martin County rural, KY | 11,943 |
| Olive Hill, KY | 1,599 |
| Paintsville, KY | 3,459 |
| Raceland, KY | 2,424 |
| Russell, KY | 3,380 |
| Sandy Hook, KY | 675 |
| South Shore, KY | 1,122 |
| Warfield, KY | 269 |
| Westwood, KY | 4,746 |
| Worthington, KY | 1,609 |
| Wurtland, KY | 995 |
| Albany village, OH | 828 |
| Athalia village, OH | 373 |
| Beaver village, OH | 449 |
| Burlington, OH | 2,676 |
| Centerville village, OH | 103 |
| Chesapeake village, OH | 745 |
| Cheshire village, OH | 132 |
| Clarktown, OH | 958 |
| Coal Grove village, OH | 2,165 |
| Coalton village, OH | 479 |
| Crown City village, OH | 413 |
| Franklin Furnace, OH | 1,660 |
| Friendship, OH | 351 |
| Gallia County rural, OH | 25,418 |
| Gallipolis village, OH | 3,641 |
| Hamden village, OH | 879 |
| Hanging Rock village, OH | 221 |
| Ironton, OH | 11,129 |
| Jackson, OH | 6,397 |
| Jackson County rural, OH | 19,135 |
| Kanauga, OH | 175 |
| Lawrence County rural, OH | 40,609 |
| Lucasville, OH | 2,757 |
| McDermott, OH | 434 |
| Meigs County rural, OH | 17,029 |
| Middleport village, OH | 2,530 |
| Minford, OH | 693 |
| New Boston village, OH | 2,272 |
| Oak Hill village, OH | 1,551 |
| Pomeroy village, OH | 1,852 |
| Portsmouth, OH | 20,226 |
| Proctorville village, OH | 574 |
| Racine village, OH | 675 |
| Rio Grande village, OH | 830 |
| Rosemount, OH | 2,112 |
| Rutland village, OH | 393 |
| Scioto County rural, OH | 36,257 |
| Sciotodale, OH | 1,081 |
| South Point village, OH | 3,958 |
| South Webster village, OH | 866 |
| Stockdale, OH | 135 |
| Syracuse village, OH | 826 |
| Tuppers Plains, OH | 465 |
| Vinton village, OH | 222 |
| Wellston, OH | 5,663 |
| West Portsmouth, OH | 3,149 |
| Wheelersburg, OH | 6,437 |
| Wilkesville village, OH | 149 |
| Zaleski village, OH | 278 |
| Accoville, WV | 574 |
| Alum Creek, WV | 1,749 |
| Amherstdale, WV | 350 |
| Apple Grove, WV | 204 |
| Bancroft town, WV | 587 |
| Barboursville village, WV | 3,964 |
| Belle town, WV | 1,260 |
| Big Chimney, WV | 627 |
| Big Creek, WV | 237 |
| Boone County rural, WV | 18,947 |
| Bruno, WV | 544 |
| Buffalo town, WV | 1,236 |
| Cabell County rural, WV | 29,337 |
| Calhoun County rural, WV | 7,066 |
| Cedar Grove town, WV | 997 |
| Ceredo, WV | 1,450 |
| Chapmanville town, WV | 1,256 |
| Charleston, WV | 51,400 |
| Chattaroy, WV | 756 |
| Chauncey, WV | 283 |
| Chelyan, WV | 776 |
| Chesapeake town, WV | 1,554 |
| Clendenin town, WV | 1,227 |
| Coal Fork, WV | 1,233 |
| Comfort, WV | 306 |
| Cross Lanes, WV | 9,995 |
| Crum, WV | 182 |
| Culloden, WV | 3,061 |
| Danville town, WV | 691 |
| Delbarton town, WV | 579 |
| Dunbar, WV | 7,907 |
| East Bank town, WV | 959 |
| Eleanor town, WV | 1,518 |
| Elkview, WV | 1,222 |
| Fort Gay town, WV | 705 |
| Gallipolis Ferry, WV | 817 |
| Gilbert Creek, WV | 1,090 |
| Gilbert town, WV | 450 |
| Glasgow town, WV | 905 |
| Greenview, WV | 378 |
| Hamlin town, WV | 1,142 |
| Handley town, WV | 349 |
| Hartford City town, WV | 614 |
| Harts, WV | 656 |
| Henderson town, WV | 271 |
| Henlawson, WV | 442 |
| Holden, WV | 876 |
| Hometown, WV | 668 |
| Huntington, WV | 49,138 |
| Hurricane, WV | 6,284 |
| Jackson County rural, WV | 22,083 |
| Jefferson, WV | 676 |
| Justice, WV | 412 |
| Kanawha County rural, WV | 64,925 |
| Kenova, WV | 3,216 |
| Kermit town, WV | 406 |
| Kistler, WV | 528 |
| Lavalette, WV | 1,073 |
| Leon town, WV | 158 |
| Lesage, WV | 1,358 |
| Lincoln County rural, WV | 17,399 |
| Logan, WV | 1,779 |
| Logan County rural, WV | 20,470 |
| Madison, WV | 3,076 |
| Mallory, WV | 1,654 |
| Man town, WV | 759 |
| Marmet, WV | 1,503 |
| Mason County rural, WV | 18,382 |
| Mason town, WV | 968 |
| Matewan town, WV | 499 |
| McConnell, WV | 514 |
| Milton town, WV | 2,423 |
| Mingo County rural, WV | 18,875 |
| Mitchell Heights town, WV | 323 |
| Monaville, WV | 309 |
| Mount Gay-Shamrock, WV | 1,779 |
| Neibert, WV | 183 |
| New Haven town, WV | 1,560 |
| Nitro, WV | 7,178 |
| Omar, WV | 552 |
| Pea Ridge, WV | 6,650 |
| Pinch, WV | 3,262 |
| Poca town, WV | 974 |
| Point Pleasant, WV | 4,350 |
| Pratt town, WV | 602 |
| Prichard, WV | 527 |
| Putnam County rural, WV | 28,743 |
| Racine, WV | 256 |
| Rand, WV | 1,631 |
| Ravenswood, WV | 3,876 |
| Red Jacket, WV | 581 |
| Reedy town, WV | 182 |
| Ripley, WV | 3,252 |
| Roane County rural, WV | 12,422 |
| Robinette, WV | 663 |
| Rossmore, WV | 301 |
| Salt Rock, WV | 388 |
| Sarah Ann, WV | 345 |
| Shrewsbury, WV | 652 |
| Sissonville, WV | 4,028 |
| South Charleston, WV | 13,450 |
| Spencer, WV | 2,322 |
| St. Albans, WV | 11,044 |
| Stollings, WV | 316 |
| Switzer, WV | 595 |
| Sylvester town, WV | 160 |
| Teays Valley, WV | 13,175 |
| Twilight, WV | 90 |
| Upper Falls, WV | 3,701 |
| Van, WV | 211 |
| Verdunville, WV | 687 |
| Wayne County rural, WV | 33,915 |
| Wayne town, WV | 1,413 |
| West Hamlin town, WV | 774 |
| West Logan town, WV | 424 |
| Whitesville town, WV | 514 |
| Williamson, WV | 3,191 |
| Winfield town, WV | 2,301 |
| | |
| Total | 983,192 |
